ACTIVITY activity spectrum | association with Pen/Strep | cytotoxicity

 

ANTIMICROBIAL ACTIVITY SPECTRUM OF FUNGIN:

The in vitro antimicrobial activity of Fungin tested against different bacteria, yeasts and fungi is reported in the following table.
To eliminate yeast and fungal contamination, using Fungin at 50 µg/ml for 5-10 days is advised.
To prevent yeast and fungal contaminations in cell culture, using routinely Fungin at 10µg/ml is advised.

Resistant
 
Resistant
 
Sensitive
from 1 to 50µg/ml
Slow growing microbial contaminants
Fast growing microbial contaminants
 
 
 
Mollicutes:
mycoplasmas and acholeplasmas
 
Bacteria:
Gram + and Gram – bacteria

Yeasts:
strains from ATCC or isolated from clinical samples

Candida albicans 49637
Candida albicans 49774
Candida parapsilosis
Saccharomyces cerevisiae
   ATCC 32119

Molds:
isolated from environment

Aspergillus oryzae
Chrysosporium spp
Trichoderma reesei
Thermoascus aurantiacus

 

ASSOCIATION with PEN / STREP :

Fungin is compatible with penicillin / streptomycin solutions. Fungin expands the activity spectrum of penicillin / streptomycin against yeasts and most filamentous fungi.

 

CYTOTOXICITY :

To date, no Fungin toxicity to cell-lines treated at 10µg/ml has been reported.

Examples of cell lines propagated in Fungin-supplemented culture media:

Cell lines propagated with a permanent adjonction of Fungin at 10 µg/ml
293 Transformed primary embryonal kidney Human
HepG2 Hepatocellular carcinoma Human
HeLa Epitheloid carcinoma, cervix Human
K562 Chronic myelogenous leukemia Human
Jurkat Acute T cell leukemia Human
CHO Ovary Hamster
PC 1.0 Pancreas adenocarcinoma Hamster
Raw 264.7 Monocyte-macrophage Mouse
C26 Colon adenocarcinoma Mouse
C2C12 Muscle myoblast Mouse
B16 Melanoma Mouse
C6 Glial tumor cell Rat

No Fungin toxicity has been reported for cell-lines treated at 50µg/ml.

At higher concentrations, like any other polyene antibiotic, Fungin may induce cytoxicity. Nevertheless, it has been demonstrated that pimaricin was the least toxic polyene compared to mediocidin, amphotericin B deoxycholate and filipin as indicated by 24-hour survival, 72-hour viability, and growth rate of cells (B1, B82 and RAG) (1).

  1. Fisher PB, Bryson V, Schaffner CP. (1978). Polyene macrolide antibiotic cytotoxicity and membrane permeability alterations. I. Comparative effects of four classes of polyene macrolides on mammalian cells. J Cell Physiol 97: 345-51